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J Agric Food Chem. 2019 Feb 13;67(6):1702-1710. doi: 10.1021/acs.jafc.8b07166. Epub 2019 Feb 5.

Antioxidant and Anti-Inflammatory Activities of Buffalo Milk δ-Valerobetaine.

Author information

1
Department of Precision Medicine , University of Campania "L. Vanvitelli" , L. De Crecchio 7 , 80138 Naples , Italy.
2
Istituto Zooprofilattico Sperimentale del Mezzogiorno , 80055 Portici, Naples , Italy.
3
Department of Veterinary Medicine and Animal Production , Federico II University , 80137 Naples , Italy.
4
Tecnologica Research Institute , Biomedical Section , 88900 Crotone , Italy.

Abstract

δ-Valerobetaine (δVB), a constitutive metabolite of ruminant milk, is produced in the rumen from free dietary Nε- trimethyllysine occurring ubiquitously in vegetable kingdom. The biological role of δVB is poorly known. Here, the antioxidant and anti-inflammatory potential of buffalo milk δVB was tested in vitro during high-glucose (HG)-induced endothelial damage. Results indicated that δVB (0.5 mM) ameliorated the HG cytotoxicity (0.57 ± 0.02 vs 0.41 ± 0.018 O.D. ( P < 0.01). Interestingly, buffalo milk extracts enriched with δVB showed improved significant efficacy in decreasing reactive oxygen species, lipid peroxidation, and cytokine release during HG treatment compared to milk extracts alone ( P < 0.05). It is noteworthy that δVB reduced the HG-activated inflammatory signal by modulating SIRT1 (0.96 ± 0.05 vs 0.85 ± 0.04 AU), SIRT6 (0.82 ± 0.04 vs 0.61 ± 0.03 AU), and NF-κB (0.85 ± 0.03 vs 1.23 ± 0.03 AU) ( P < 0.05). On the whole, our data show the first evidence of δVB efficacy in reducing endothelial oxidative stress and inflammation, suggesting a potential role of this betaine as a novel dietary compound with health-promoting properties.

KEYWORDS:

SIRT1; SIRT6; buffalo milk; hyperglycaemia; reactive oxygen species; δ-valerobetaine

PMID:
30661355
DOI:
10.1021/acs.jafc.8b07166

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