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BMC Genomics. 2019 Jan 18;20(1):59. doi: 10.1186/s12864-018-5299-0.

High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

Author information

1
Center for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
2
Regensburg Centre for Interventional Immunology (RCI) and University Medical Center, Regensburg, Germany.
3
Center for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden. robert.mansson@ki.se.
4
Hematology Center, Karolinska University Hospital, Stockholm, Sweden. robert.mansson@ki.se.

Abstract

BACKGROUND:

Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.

RESULTS:

We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.

CONCLUSIONS:

The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

KEYWORDS:

ChIP-seq; ChIPmentation; Chromatin immunoprecipitation; Epigenetics; High-throughput genomics

PMID:
30658577
PMCID:
PMC6339279
DOI:
10.1186/s12864-018-5299-0
[Indexed for MEDLINE]
Free PMC Article

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