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Methods Mol Biol. 2019;1919:59-72. doi: 10.1007/978-1-4939-9007-8_5.

Chemically Defined Neural Conversion of Human Pluripotent Stem Cells.

Author information

1
Stem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
2
Stem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA. ilyas.singec@nih.gov.

Abstract

Human pluripotent stem cells (hPSCs) are characterized by their ability to self-renew and differentiate into any cell type of the human body. To fully utilize the potential of hPSCs for translational research and clinical applications, it is critical to develop rigorous cell differentiation protocols under feeder-free conditions that are efficient, reproducible, and scalable for high-throughput projects. Focusing on neural conversion of hPSCs, here we describe robust small molecule-based procedures that generate neural stem cells (NSCs) in less than a week under chemically defined conditions. These protocols can be used to dissect the mechanisms of neural lineage entry and to further develop systematic protocols that produce the cellular diversity of the central nervous system at industrial scale.

KEYWORDS:

Cell differentiation; Coating substrate; Culture medium; Embryonic stem cell; Induced pluripotent stem cell; Neural induction; Pathway inhibition; Pluripotency; Small molecules

PMID:
30656621
DOI:
10.1007/978-1-4939-9007-8_5
[Indexed for MEDLINE]

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