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Methods Mol Biol. 2019;1919:25-41. doi: 10.1007/978-1-4939-9007-8_3.

Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury.

Khazaei M1, Ahuja CS1,2,3, Rodgers CE1,2, Chan P1,2, Fehlings MG4,5,6,7,8.

Author information

1
Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada.
2
Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
3
Division of Neurosurgery, University of Toronto, Toronto, ON, Canada.
4
Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
5
Institute of Medical Science, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
6
Division of Neurosurgery, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
7
Spinal Program, Toronto Western Hospital, University Health Network, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.
8
Faculty of Medicine, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

Abstract

In this chapter, we first describe two interchangeable protocols optimized in our lab for deriving definitive neuronal progenitor cells from human pluripotent stem cells (hPSCs). The resultant NPCs can then be propagated and differentiated to produce differing proportions of neurons, oligodendrocytes, and astrocytes as required for in vitro cell culture studies or in vivo transplantation. Following these protocols, we explain the method for transplanting these cells into the rat model of spinal cord injury (SCI).

KEYWORDS:

Cell transplantation; Neural progenitor cells; Pluripotent stem cells; Spinal cord injury

PMID:
30656619
DOI:
10.1007/978-1-4939-9007-8_3
[Indexed for MEDLINE]

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