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Nanoscale Res Lett. 2019 Jan 17;14(1):26. doi: 10.1186/s11671-018-2834-5.

Exposure to Titanium Dioxide Nanoparticles During Pregnancy Changed Maternal Gut Microbiota and Increased Blood Glucose of Rat.

Mao Z1,2,3, Li Y2,3, Dong T2,3, Zhang L1, Zhang Y2,3, Li S2,3, Hu H1, Sun C4, Xia Y5,6.

Author information

1
Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, 213003, Jiangsu, China.
2
State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 101 Longmian Road, Nanjing, 211100, China.
3
Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211100, China.
4
Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, 213003, Jiangsu, China. suncaifeng04@126.com.
5
State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 101 Longmian Road, Nanjing, 211100, China. yankaixia@njmu.edu.cn.
6
Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211100, China. yankaixia@njmu.edu.cn.

Abstract

Titanium dioxide nanoparticles (TiO2 NPs) were used worldwide for decades, and pregnant women are unable to avoid exposing to them. Studies revealed that TiO2 NPs could kill many kinds of bacteria, but whether they would affect the composition of gut microbiota, especially during pregnancy, was seldom reported. And, what adverse effects may be brought to pregnant females was also unknown. In this study, we established the prenatal exposure model of rats to explore the effects of TiO2 NPs on gut microbiota. We observed an increasing trend, but not a significant change of alpha-diversity among control and exposure groups at gestation day (GD) 10 and GD 17 during normal pregnancy process. Each different time point had unique gut microbiota operational taxonomic units (OTUs) characteristics. The abundance of Ellin6075 decreased at GD 10 and GD 17, Clostridiales increased at GD 10, and Dehalobacteriaceae decreased at GD 17 after TiO2 NPs exposure. Further phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) prediction indicated that the type 2 diabetes mellitus related genes were enhanced, and taurine metabolism was weakened at the second-trimester. Further study showed that the rats' fasting blood glucose levels significantly increased at GD 10 (P < 0.05) and GD 17 (P < 0.01) after exposure. Our study pointed out that TiO2 NPs induced the alteration of gut microbiota during pregnancy and increased the fasting blood glucose of pregnant rats, which might increase the potential risk of gestational diabetes of pregnant women.

KEYWORDS:

Gut microbiota; Increased fasting blood glucose; Pregnancy exposure; TiO2 NPs

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