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BMC Genomics. 2019 Jan 16;20(1):46. doi: 10.1186/s12864-018-5266-9.

Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp.

Author information

1
Department of Fish Diseases, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100, Pulawy, Poland. reichert@piwet.pulawy.pl.
2
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.
3
Department of Plant Molecular Biology, Institute of Experimental Plant Biology and Biotechnology, University of Warsaw, 02-096, Warsaw, Poland.
4
Department of Fish Diseases, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100, Pulawy, Poland.

Abstract

BACKGROUND:

The mechanism of latency and the ability of the cyprinid herpesvirus 3 (CyHV-3) to establish life-long infections in carp remains poorly understood. To explain the role of miRNAs in this process we applied a range of molecular tools including high-throughput sequencing of RNA libraries constructed from the blood samples of infected fish followed by bioinformatic and functional analyses which show that CyHV-3 profoundly influences the expression of host miRNAs in vivo.

RESULTS:

We demonstrated the changed expression of 27 miRNAs in the clinical phase and 5 in the latent phase of infection. We also identified 23 novel, not previously reported sequences, from which 8 showed altered expressions in control phase, 10 in clinical phase and 5 in latent phase of infection.

CONCLUSIONS:

The results of our analysis expand the knowledge of common carp microRNAs engaged during CyHV-3 infection and provide a useful basis for the further study of the mechanism of CyHV-3 induced pathology.

KEYWORDS:

Cellular miRNA expression; Common carp; Herpesvirus; Infection; KHV

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