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J Cell Biochem. 2019 Jun;120(6):10678-10687. doi: 10.1002/jcb.28358. Epub 2019 Jan 16.

MicroRNA-145 performs as a tumor suppressor in human esophageal squamous cell carcinoma by targeting phospholipase C epsilon 1.

Author information

1
Department of Nephrology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, P.R. China.
2
Department of Cardiothoracic and Vascular Surgery, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, P.R. China.
3
Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China.
4
Center for Health Examination, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, P.R. China.

Abstract

Esophageal squamous cell carcinoma (ESCC) is the leading pathologic type in China. miR-145 has been reported to be downregulated in multiple tumors. This study was aimed to investigate the role of miR-145 in ESCC. miR-145 expression was investigated in 65 ESCC samples as well as four ESCC cell lines by quantitative real-time polymerase chain reaction (qRT-PCR). Targetscan 6.2 website (http://www.targetscan.org/) was used to predict the targets of miR-145. Expression of phospholipase C epsilon 1 (PLCE1) messenger RNA and protein was detected by qRT-PCR or Western blot. MTT and wound healing assay were conducted to explore the effects of miR-145 on the proliferation and migration of ESCC cell lines, respectively. miR-145 was significantly decreased in ESCC tissues. An inverse correlation between miR-145 and invasion depth and TNM stage were observed. PLCE1 was a direct target of miR-145, and the expression of PLCE1 was inversely correlated with miR-145 expression in ESCC tissues. In addition, overexpression of miR-145 suppressed cell proliferation and migration in ESCC cells. The enforced expression of PLCE1 partially reversed the suppressive effect of miR-145. These results prove that miR-145 may perform as a tumor suppressor in ESCC by targeting PLCE1.

KEYWORDS:

esophageal squamous cell carcinoma; microRNA-145; migration; phospholipase C epsilon 1; proliferation

PMID:
30652343
DOI:
10.1002/jcb.28358

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