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Cells. 2019 Jan 15;8(1). pii: E53. doi: 10.3390/cells8010053.

Dystrophin Deficiency Leads to Genomic Instability in Human Pluripotent Stem Cells via NO Synthase-Induced Oxidative Stress.

Author information

1
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. sarka.jelinkova89@gmail.com.
2
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. sarka.jelinkova89@gmail.com.
3
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. petrfoj@hotmail.com.
4
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. aneta.baumeisterova@gmail.com.
5
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. aneta.baumeisterova@gmail.com.
6
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. aleksandra.vilotic@gmail.com.
7
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. zerzankova.lenka@gmail.com.
8
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. peslmartin@gmail.com.
9
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. peslmartin@gmail.com.
10
1st department of Internal Medicine-Cardioangiology, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. peslmartin@gmail.com.
11
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. t.jurakova@gmail.com.
12
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. kruta.miriama@gmail.com.
13
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. vrbsky.jan@fnusa.cz.
14
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. gaillyova@fnbrno.cz.
15
Department of Clinical Genetics, University hospital Brno, 613 00 Brno, Czech Republic. gaillyova@fnbrno.cz.
16
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. Valaskova.Iveta@fnbrno.cz.
17
Department of Clinical Genetics, University hospital Brno, 613 00 Brno, Czech Republic. Valaskova.Iveta@fnbrno.cz.
18
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. ivan.frak@gmail.com.
19
PhyMedExp, INSERM, University of Montpellier, CNRS, 342 95 Montpellier CEDEX 5, France. alain.lacampagne@inserm.fr.
20
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. giaforte@gmail.com.
21
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. petr.dvorak@rect.muni.cz.
22
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. petr.dvorak@rect.muni.cz.
23
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. albano.meli@inserm.fr.
24
PhyMedExp, INSERM, University of Montpellier, CNRS, 342 95 Montpellier CEDEX 5, France. albano.meli@inserm.fr.
25
Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. vrotrekl@med.muni.cz.
26
International Clinical Research Center ICRC, St. Anne's University Hospital Brno, 602 00 Brno, Czech Republic. vrotrekl@med.muni.cz.

Abstract

Recent data on Duchenne muscular dystrophy (DMD) show myocyte progenitor's involvement in the disease pathology often leading to the DMD patient's death. The molecular mechanism underlying stem cell impairment in DMD has not been described. We created dystrophin-deficient human pluripotent stem cell (hPSC) lines by reprogramming cells from two DMD patients, and also by introducing dystrophin mutation into human embryonic stem cells via CRISPR/Cas9. While dystrophin is expressed in healthy hPSC, its deficiency in DMD hPSC lines induces the release of reactive oxygen species (ROS) through dysregulated activity of all three isoforms of nitric oxide synthase (further abrev. as, NOS). NOS-induced ROS release leads to DNA damage and genomic instability in DMD hPSC. We were able to reduce both the ROS release as well as DNA damage to the level of wild-type hPSC by inhibiting NOS activity.

KEYWORDS:

DMD; NO synthases; ROS; dystrophin; genome stability; pluripotent stem cells

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