Format

Send to

Choose Destination
Int J Mol Sci. 2019 Jan 15;20(2). pii: E328. doi: 10.3390/ijms20020328.

De Novo Assembly and Discovery of Genes That Involved in Drought Tolerance in the Common Vetch.

Author information

1
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zyq80842@hotmail.com.
2
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. sicauliuqiuxu@163.com.
3
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. xuwenzhi_herb@126.com.
4
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zjhu-11@163.com.
5
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. wxowdj@126.com.
6
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. nieganggrass@hotmail.com.
7
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yaolisfri@163.com.
8
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. wang.hongde163@163.com.
9
Institute of Soil and Fertilizer Research, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. lcw-11@163.com.

Abstract

The common vetch (Vicia sativa) is often used as feed for livestock because of its high nutritional value. However, drought stress reduces forage production through plant damage. Here, we studied the transcriptional profiles of common vetch exposed to drought in order to understand the molecular mechanisms of drought tolerance in this species. The genome of the common vetch has not been sequenced, therefore we used Illumina sequencing to generate de novo transcriptomes. Nearly 500 million clean reads were used to generate 174,636 transcripts, including 122,299 unigenes. In addition, 5313 transcription factors were identified and these transcription factors were classified into 79 different gene families. We also identified 11,181 SSR loci from di- to hexa-nucleotides whose repeat number was greater than five. On the basis of differentially expressed genes, Gene Ontology analysis identified many drought-relevant categories, including "oxidation-reduction process", "lipid metabolic process" and "oxidoreductase activity". In addition to these, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified pathways, such as "Plant hormone signal transduction", "Glycolysis/Gluconeogenesis" and "Phenylpropanoid biosynthesis", as differentially expressed in the plants exposed to drought. The expression results in this study will be useful for further extending our knowledge on the drought tolerance of common vetch.

KEYWORDS:

common vetch; differentially expressed genes; drought; next-generation sequencing

PMID:
30650531
PMCID:
PMC6359484
DOI:
10.3390/ijms20020328
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center