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Nat Med. 2019 Feb;25(2):263-269. doi: 10.1038/s41591-018-0296-z. Epub 2019 Jan 14.

Biomimetic 3D-printed scaffolds for spinal cord injury repair.

Author information

1
Department of Neuroscience, University of California San Diego, La Jolla, CA, USA. ykoffler@ucsd.edu.
2
Department of NanoEngineering, University of California San Diego, La Jolla, CA, USA.
3
Department of Anesthesiology, University of California San Diego, La Jolla, CA, USA.
4
Department of Neuroscience, University of California San Diego, La Jolla, CA, USA.
5
Mechanical Engineering Department, University of Michigan, Ann Arbor, MI, USA.
6
Department of NanoEngineering, University of California San Diego, La Jolla, CA, USA. chen168@eng.ucsd.edu.
7
Department of Neuroscience, University of California San Diego, La Jolla, CA, USA. mtuszynski@ucsd.edu.
8
Veterans Affairs Medical Center, San Diego, CA, USA. mtuszynski@ucsd.edu.

Abstract

Current methods for bioprinting functional tissue lack appropriate biofabrication techniques to build complex 3D microarchitectures essential for guiding cell growth and promoting tissue maturation1. 3D printing of central nervous system (CNS) structures has not been accomplished, possibly owing to the complexity of CNS architecture. Here, we report the use of a microscale continuous projection printing method (μCPP) to create a complex CNS structure for regenerative medicine applications in the spinal cord. μCPP can print 3D biomimetic hydrogel scaffolds tailored to the dimensions of the rodent spinal cord in 1.6 s and is scalable to human spinal cord sizes and lesion geometries. We tested the ability of µCPP 3D-printed scaffolds loaded with neural progenitor cells (NPCs) to support axon regeneration and form new 'neural relays' across sites of complete spinal cord injury in vivo in rodents1,2. We find that injured host axons regenerate into 3D biomimetic scaffolds and synapse onto NPCs implanted into the device and that implanted NPCs in turn extend axons out of the scaffold and into the host spinal cord below the injury to restore synaptic transmission and significantly improve functional outcomes. Thus, 3D biomimetic scaffolds offer a means of enhancing CNS regeneration through precision medicine.

PMID:
30643285
PMCID:
PMC6559945
DOI:
10.1038/s41591-018-0296-z
[Indexed for MEDLINE]
Free PMC Article

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