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Nat Commun. 2019 Jan 14;10(1):191. doi: 10.1038/s41467-018-08124-7.

Strigolactone perception and deactivation by a hydrolase receptor DWARF14.

Author information

1
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan. yoshiya@meiji.ac.jp.
2
RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. yoshiya@meiji.ac.jp.
3
Jack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA. yoshiya@meiji.ac.jp.
4
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA. yoshiya@meiji.ac.jp.
5
Department of Agricultural Chemistry, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan. yoshiya@meiji.ac.jp.
6
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan.
7
Graduate School of Agricultural and Life Science, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657, Japan.
8
Graduates School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan.
9
Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan.
10
Department of Animal, Plant and Soil Sciences AgriBio, Centre for AgriBioscience, La Trobe University, 5 Ring Road Bundoora, Melbourne, VIC, 3086, Australia.
11
Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Mozume, Muko, Kyoto, 617-0001, Japan.
12
RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.
13
Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193, Japan.
14
Jack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
15
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
16
Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan.
17
Structural Biology Laboratory, Nara Institute of Science and Technology, 8916-5 Takeyama, Ikoma, Nara, 630-0192, Japan.
18
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan. shinjiro@scl.kyoto-u.ac.jp.
19
RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. shinjiro@scl.kyoto-u.ac.jp.
20
Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan. shinjiro@scl.kyoto-u.ac.jp.

Abstract

The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.

PMID:
30643123
PMCID:
PMC6331613
DOI:
10.1038/s41467-018-08124-7
[Indexed for MEDLINE]
Free PMC Article

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