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BMC Genomics. 2019 Jan 14;20(1):37. doi: 10.1186/s12864-018-5410-6.

MicroRNA expression profile of HCT-8 cells in the early phase of Cryptosporidium parvum infection.

Wang C1,2, Liu L1,2, Zhu H3, Zhang L1,2, Wang R1,2, Zhang Z1,2, Huang J1,2, Zhang S1,2, Jian F1,2, Ning C1,2, Zhang L4,5.

Author information

1
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
2
International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, 450002, People's Republic of China.
3
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, People's Republic of China.
4
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. zhanglx8999@gmail.com.
5
International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, 450002, People's Republic of China. zhanglx8999@gmail.com.

Abstract

BACKGROUND:

Cryptosporidium parvum is an important zoonotic parasitic disease worldwide, but the molecular mechanisms of the host-parasite interaction are not fully understood. Noncoding microRNAs (miRNAs) are considered key regulators of parasitic diseases. Therefore, we used microarray, qPCR, and bioinformatic analyses to investigate the intestinal epithelial miRNA expression profile after Cryptosporidium parvum infection.

RESULTS:

Twenty miRNAs were differentially expressed after infection (four upregulated and 16 downregulated). Further analysis of the differentially expressed miRNAs revealed that many important cellular responses were triggered by Cryptosporidium parvum infection, including cell apoptosis and the inflammatory and immune responses.

CONCLUSIONS:

This study demonstrates for the first time that the miRNA expression profile of human intestinal epithelium cells is altered by C. parvum infection. This dysregulation of miRNA expression may contribute to the regulation of host biological processes in response to C. parvum infection, including cell apoptosis and the immune responses. These results provide new insight into the regulatory mechanisms of host miRNAs during cryptosporidiosis, which may offer potential targets for future C. parvum control strategies.

KEYWORDS:

Cryptosporidium parvum; Infection; Intestinal epithelium; MicroRNA

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