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BMC Evol Biol. 2019 Jan 14;19(1):22. doi: 10.1186/s12862-018-1326-7.

Improved inference of site-specific positive selection under a generalized parametric codon model when there are multinucleotide mutations and multiple nonsynonymous rates.

Author information

1
Department of Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4J1, Canada.
2
Department of Mathematics & Statistics, Dalhousie University, Halifax, Nova Scotia, B3H 4J1, Canada.
3
Department of Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4J1, Canada. j.bielawski@dal.ca.
4
Department of Mathematics & Statistics, Dalhousie University, Halifax, Nova Scotia, B3H 4J1, Canada. j.bielawski@dal.ca.
5
Centre Comparative Genomics and Evolutionary Bioinformatics (CGEB) at Dalhousie University, Halifax, Canada. j.bielawski@dal.ca.

Abstract

BACKGROUND:

An excess of nonsynonymous substitutions, over neutrality, is considered evidence of positive Darwinian selection. Inference for proteins often relies on estimation of the nonsynonymous to synonymous ratio (ω = dN/dS) within a codon model. However, to ease computational difficulties, ω is typically estimated assuming an idealized substitution process where (i) all nonsynonymous substitutions have the same rate (regardless of impact on organism fitness) and (ii) instantaneous double and triple (DT) nucleotide mutations have zero probability (despite evidence that they can occur). It follows that estimates of ω represent an imperfect summary of the intensity of selection, and that tests based on the ω > 1 threshold could be negatively impacted.

RESULTS:

We developed a general-purpose parametric (GPP) modelling framework for codons. This novel approach allows specification of all possible instantaneous codon substitutions, including multiple nonsynonymous rates (MNRs) and instantaneous DT nucleotide changes. Existing codon models are specified as special cases of the GPP model. We use GPP models to implement likelihood ratio tests for ω > 1 that accommodate MNRs and DT mutations. Through both simulation and real data analysis, we find that failure to model MNRs and DT mutations reduces power in some cases and inflates false positives in others. False positives under traditional M2a and M8 models were very sensitive to DT changes. This was exacerbated by the choice of frequency parameterization (GY vs. MG), with rates sometimes > 90% under MG. By including MNRs and DT mutations, accuracy and power was greatly improved under the GPP framework. However, we also find that over-parameterized models can perform less well, and this can contribute to degraded performance of LRTs.

CONCLUSIONS:

We suggest GPP models should be used alongside traditional codon models. Further, all codon models should be deployed within an experimental design that includes (i) assessing robustness to model assumptions, and (ii) investigation of non-standard behaviour of MLEs. As the goal of every analysis is to avoid false conclusions, more work is needed on model selection methods that consider both the increase in fit engendered by a model parameter and the degree to which that parameter is affected by un-modelled evolutionary processes.

KEYWORDS:

Codon frequencies; Codon model; False positives; G-series models; Likelihood ratio test; M-series models; Model misspecification; Multiple nonsynonymous rates; Multiple nucleotide mutations; Positive selection; Protein evolution

PMID:
30642241
PMCID:
PMC6332903
DOI:
10.1186/s12862-018-1326-7
[Indexed for MEDLINE]
Free PMC Article

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