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Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Nov;36(11):1358-1363.

[Effect of Qihuang Decoction on the Intestinal Mucosal Immunologic Barrier of Rats after Gastric Resection].

[Article in Chinese]

Abstract

Objective To observe the effect of Qihuang Decoction (QHD) on the intestinal muco- sal immunologic barrier of rats after gastric resection. Methods Sixty Wistar rats were randomly divided into the sham-operation group, the model group, and the QHD group, 20 in each group. Rats in the model group and the QHD group received gastric resection. Intestinal dripping Nutrison (an intacted protein en- teral nutrition powder). was given to rats in the model group after resection. Intestinal dripping Nutrison and QHD was given to rats in the QHD group. Rats in the sham-operation group only received abdominal midsection and suture. They ate and drank normally with no drug or nutrition intervention. After one-week intervention, Peyer's patches (PPs) , lamina propria lymphocytes (LPLs) , intraepithelial lymphocytes (IELs) , secretory IgA (sIgA) were isolated from rat small intestine. Ratios of αβT cell antigen receptor (αβTCR) -cluster of differentiation 3 positive (CD3+ ) , cluster of differentiation 4 positive (CD4 +) , clus- ter of differentiation 8 positive (CD8 +) , counts of IgA + B lymphocytes, and contents of intestinal mucosa sIgA were detected using flow cytometry, immunohistochemistry, and double antibody-PEG radioimmu- noassay. Results Compared with the sham-operation group, contents of intestinal mucosa sIgA, counts of IgA +B lymphocytes in PPs, and counts of lgA B lymphocytes in LPLs all decreased (P <0. 01) ; CD3 + and CD8 +T ratios in IELs, CD3 +, CD4 +, and CD8 +ratios in LPLs; CD3 + and CD4 + ratios in PPs de- creased (P <0. 01, P <0. 05) in the model group. Compared with the model group, contents of intestinal mucosa slgA, counts of IgA+B lymphocytes in PPs, and counts of IgA + B lymphocytes in LPLs all in- creased in the QHD group (P <0. 01 , P <0. 05) ; CD3 + and CD8 +T ratios in IELs, CD3 + and CD4 + ratios in LPLs, CD3+ and CD4+ ratios in PPs increased in the QHD group (P <0. 01, P <0. 05). Conclusion QHD could promote differentiation and multiplication of CD3 + , CD4 +, CD8 + T, and IgA + B lymphocytes in the intestinal mucosal immunologic barrier, increase contents of intestinal mucosa slgA, and promote the recovery of intestinal mucosal immunologic barrier of gastric resection rats.

PMID:
30641631
[Indexed for MEDLINE]

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