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J Glob Antimicrob Resist. 2019 Jan 10. pii: S2213-7165(19)30009-8. doi: 10.1016/j.jgar.2019.01.002. [Epub ahead of print]

ESBL-producing Enterobacteriaceae from animal origins and wastewater in Tunisia: first detection of O25b-B23-CTX-M-27-ST131 Escherichia coli and CTX-M-15-OXA-204-producing Citrobacter freundii from wastewater.

Author information

1
Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisie; Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj-Cédria, BP 273, 8020, Soliman, Tunisie.
2
Université de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis 1006, Tunisie; Université de Tunis El Manar, Faculté de Médecine de Tunis, Laboratoire de résistance aux antibiotiques LR99ES09, Tunisie. Electronic address: salahtoumi_mohamed@yahoo.com.
3
University Hospital Virgen Macarena, Seville, Spain; Microbiology Department of School of Medicine, University of Seville, Seville, Spain.
4
University Hospital Virgen Macarena, Seville, Spain.
5
Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj-Cédria, BP 273, 8020, Soliman, Tunisie.

Abstract

OBJECTIVES:

The aim of this study was to isolate and to characterize ESBL-producing Enterobacteriaceae from animals and wastewater from Tunisia.

MATERIALS AND METHODS:

ESBL-producing Enterobacteriaceae were investigated from 123 samples of wastewater, 140 healthy animal's feces samples (poultry, sheep, goats, and calves) as well as raw milk from healthy cow (n=42), and goat (n=20). Antimicrobial susceptibility was tested according to the recommendation of CLSI. blaTEM, blaSHV, blaCTX-M, and blaOXA-48 genes were analyzed by PCR and sequencing. Phylogenetic groups were determined by PCR for E. coli isolates. The clonality of E. coli and K. pneumoniae isolates was determined by Xba-I-PFGE and MLST.

RESULTS:

Eighty-one E. coli, 20K. pneumoniae, 4 E. cloacae, one C. freundii and one C. brakii were collected. The blaCTX-M-1 and blaCTX-M-15genes were predominant in E. coli and K. pneumoniae isolates. E. cloacae and C. freundii isolates harbored the blaSHV-12 gene. C. braakii isolate, isolated from wastewater, carried blaCTX-M-15, blaTEM-1 and blaOXA-204 genes. E. coli isolates belonged to phylogroups A (37), B1 (25), B2 (7), and D (12). Seventy-eight E. coli isolates were typeapble by PFGE and were classified in 40 pulsotypes. The K. pneumoniae isolates belonged to 11 pulsotypes. In E. coli, the following STs were obtained: ST131, ST224, ST162, ST845, ST5204, ST69, ST141, and ST10. The K. pneumoniae isolates belonged to ST405, ST147, ST564, ST307, ST152, ST45, ST661, and ST1564.

CONCLUSION:

This is the first report of O25b-B23-CTX-M-27-ST131 E. coli isolates and C. freundii isolate carrying blaCTX-M-15, blaTEM-1 and blaOXA-204 genes in Tunisia.

KEYWORDS:

CTX-M; ESBL; bla(OXA-204)-producing C. freundii; livestock; wastewater

PMID:
30639890
DOI:
10.1016/j.jgar.2019.01.002

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