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Mol Phylogenet Evol. 2019 Apr;133:152-163. doi: 10.1016/j.ympev.2019.01.006. Epub 2019 Jan 11.

Genome-scale phylogenetics reveals a monophyletic Zoopagales (Zoopagomycota, Fungi).

Author information

1
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, United States.
2
Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
3
Department of Microbiology and Plant Pathology, University of California-Riverside, United States.
4
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.
5
United States of America Department of Energy Joint Genome Institute, Walnut Creek, CA, United States.
6
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, United States. Electronic address: tyjames@umich.edu.

Abstract

Previous genome-scale phylogenetic analyses of Fungi have under sampled taxa from Zoopagales; this order contains many predacious or parasitic genera, and most have never been grown in pure culture. We sequenced the genomes of 4 zoopagalean taxa that are predators of amoebae, nematodes, or rotifers and the genome of one taxon that is a parasite of amoebae using single cell sequencing methods with whole genome amplification. Each genome was a metagenome, which was assembled and binned using multiple techniques to identify the target genomes. We inferred phylogenies with both super matrix and coalescent approaches using 192 conserved proteins mined from the target genomes and performed ancestral state reconstructions to determine the ancestral trophic lifestyle of the clade. Our results indicate that Zoopagales is monophyletic. Ancestral state reconstructions provide moderate support for mycoparasitism being the ancestral state of the clade.

KEYWORDS:

Acaulopage tetraceros; Cochlonema odontosperma; Single cell genomics; Stylopage hadra; Zoopage; Zoophagus insidians

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