Format

Send to

Choose Destination
Nat Commun. 2019 Jan 11;10(1):141. doi: 10.1038/s41467-018-08083-z.

A gene expression map of shoot domains reveals regulatory mechanisms.

Tian C1, Wang Y2, Yu H1,2,3, He J1,4, Wang J1,2,5, Shi B1,2,6, Du Q1,2, Provart NJ7, Meyerowitz EM8, Jiao Y9,10.

Author information

1
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
2
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
3
West China Biomedical Big Data Center, West China Hospital/West China School of Medicine, and Medical Big Data Center, Sichuan University, Chengdu, 610041, China.
4
Horticultural Plant Biology and Metabolomics Center, Institute of Science and Technology, Fujian Agricultural and Forestry University, Fuzhou, Fujian, 35002, China.
5
Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
6
Laboratoire Reproduction et Developpement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, 69342, Lyon, France.
7
Department of Cell and Systems Biology, Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON, M5S 3B2, Canada.
8
Howard Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
9
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. yljiao@genetics.ac.cn.
10
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China. yljiao@genetics.ac.cn.

Abstract

Gene regulatory networks control development via domain-specific gene expression. In seed plants, self-renewing stem cells located in the shoot apical meristem (SAM) produce leaves from the SAM peripheral zone. After initiation, leaves develop polarity patterns to form a planar shape. Here we compare translating RNAs among SAM and leaf domains. Using translating ribosome affinity purification and RNA sequencing to quantify gene expression in target domains, we generate a domain-specific translatome map covering representative vegetative stage SAM and leaf domains. We discuss the predicted cellular functions of these domains and provide evidence that dome seemingly unrelated domains, utilize common regulatory modules. Experimental follow up shows that the RABBIT EARS and HANABA TARANU transcription factors have roles in axillary meristem initiation. This dataset provides a community resource for further study of shoot development and response to internal and environmental signals.

PMID:
30635575
PMCID:
PMC6329838
DOI:
10.1038/s41467-018-08083-z
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center