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Nat Commun. 2019 Jan 11;10(1):134. doi: 10.1038/s41467-018-08079-9.

Single-cell transcriptomic analysis of mouse neocortical development.

Author information

1
Department of Cell Biology and Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, 115 Mason Farm Road, Chapel Hill, NC, 27599, USA.
2
Carolina Institute for Developmental Disabilities, The University of North Carolina at Chapel Hill, Campus Box #7255,, Chapel Hill, NC, 27599, USA.
3
Department of Genetics, The University of North Carolina at Chapel Hill, Campus Box #7264,, Chapel Hill, NC, 27599, USA.
4
Department of Cell Biology and Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, 115 Mason Farm Road, Chapel Hill, NC, 27599, USA. zylka@med.unc.edu.
5
Carolina Institute for Developmental Disabilities, The University of North Carolina at Chapel Hill, Campus Box #7255,, Chapel Hill, NC, 27599, USA. zylka@med.unc.edu.

Abstract

The development of the mammalian cerebral cortex depends on careful orchestration of proliferation, maturation, and migration events, ultimately giving rise to a wide variety of neuronal and non-neuronal cell types. To better understand cellular and molecular processes that unfold during late corticogenesis, we perform single-cell RNA-seq on the mouse cerebral cortex at a progenitor driven phase (embryonic day 14.5) and at birth-after neurons from all six cortical layers are born. We identify numerous classes of neurons, progenitors, and glia, their proliferative, migratory, and activation states, and their relatedness within and across age. Using the cell-type-specific expression patterns of genes mutated in neurological and psychiatric diseases, we identify putative disease subtypes that associate with clinical phenotypes. Our study reveals the cellular template of a complex neurodevelopmental process, and provides a window into the cellular origins of brain diseases.

PMID:
30635555
PMCID:
PMC6329831
DOI:
10.1038/s41467-018-08079-9
[Indexed for MEDLINE]
Free PMC Article

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