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Neuron. 2019 Feb 6;101(3):500-513.e5. doi: 10.1016/j.neuron.2018.12.009. Epub 2019 Jan 8.

Layer-Specific Physiological Features and Interlaminar Interactions in the Primary Visual Cortex of the Mouse.

Author information

1
Neuroscience Institute, New York University, Langone Medical Center, New York, NY 10016, USA.
2
Neuroscience Institute, New York University, Langone Medical Center, New York, NY 10016, USA; Department of Neurology, Langone Medical Center, New York University, New York, NY 10016, USA; Center for Neural Science, New York University, New York, NY 10003, USA. Electronic address: gyorgy.buzsaki@nyumc.org.

Abstract

The relationship between mesoscopic local field potentials (LFPs) and single-neuron firing in the multi-layered neocortex is poorly understood. Simultaneous recordings from all layers in the primary visual cortex (V1) of the behaving mouse revealed functionally defined layers in V1. The depth of maximum spike power and sink-source distributions of LFPs provided consistent laminar landmarks across animals. Coherence of gamma oscillations (30-100 Hz) and spike-LFP coupling identified six physiological layers and further sublayers. Firing rates, burstiness, and other electrophysiological features of neurons displayed unique layer and brain state dependence. Spike transmission strength from layer 2/3 cells to layer 5 pyramidal cells and interneurons was stronger during waking compared with non-REM sleep but stronger during non-REM sleep among deep-layer excitatory neurons. A subset of deep-layer neurons was active exclusively in the DOWN state of non-REM sleep. These results bridge mesoscopic LFPs and single-neuron interactions with laminar structure in V1.

KEYWORDS:

alpha rhythm; cell types; cortical layers; current source density; laminar recordings; optogenetics; oscillations; primary visual cortex; sleep

PMID:
30635232
PMCID:
PMC6367010
DOI:
10.1016/j.neuron.2018.12.009
[Indexed for MEDLINE]
Free PMC Article

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