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J Glob Antimicrob Resist. 2019 Jan 8. pii: S2213-7165(19)30007-4. doi: 10.1016/j.jgar.2018.12.025. [Epub ahead of print]

Dissemination and characterization of extended spectrum β-lactamase, AmpC β-lactamase and metallo β-lactamase producing Escherichia coli from livestock and poultry in Northeastern India: A molecular surveillance approach.

Author information

1
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India; Department of Microbiology, Jain University, Bengaluru 560011 India.
2
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India; School of Basic and Applied Sciences, Dayananda Sagar University, Bengaluru 560078 India.
3
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India; Division of Pulmonary/Allergy/Critical care, University of Alabama at Birmingham, AL 35294, USA.
4
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India.
5
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India; International Livestock Research Institute, New Delhi 110012, India.
6
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064 India. Electronic address: brshome29@gmail.com.

Abstract

OBJECTIVES:

The study aimed to identify and characterize probable ESBL, AmpC and/or MBL producing E. colivariants circulating in the livestock and poultry environment to establish their epidemiological significance, genetic diversity, trends in antimicrobial resistance and virulence.

METHODS:

Culture method and E. coli specific multiplex PCR identified a total of 78 E. coli strains from faecal samples of healthy livestock and poultry. Antibiogram was determined by disk diffusion and MIC methods Drug resistant E. coli isolates were screened for ESBL, AmpC and MBL genes. The isolates were further characterized by plasmid replicon typing, integron assay and virulence gene analysis. Genetic diversity was assessed by RAPD and MLST.

RESULTS:

ESBL (CTXM-I, CTXM-IV, TEM), AmpC (EBC, FOX, CMY, DHA) and MBL (IMP, SIM) resistance determinants were identified in 75%, 19%, and 6% of isolates, respectively. A total of 9 plasmid replicon types were distributed among resistant E. coli strains. The most common plasmid replicon types identified were L/M and Y. Integrons were detected in 19% of E. coli isolates. RAPD analysis categorized E. coli isolates into three clusters. MLST revealed 7 different sequence types with ST10-E. coli as the most common ST.

CONCLUSIONS:

The present study demonstrated high prevalence of animals carrying potential ESBL and AmpC producing E. coli. The study emphasizes the need of rigorous surveillance in animal sector to identify the critical control points conducive to preventrapid dissemination of AMR.

KEYWORDS:

AmpC; Antimicrobial resistance; Escherichia coli; Extended Spectrum; India; Metallo; β-Lactamase

PMID:
30634056
DOI:
10.1016/j.jgar.2018.12.025

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