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Anim Biotechnol. 2019 Jan 11:1-13. doi: 10.1080/10495398.2018.1553788. [Epub ahead of print]

LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells.

Author information

1
a College of Animal Science & Veterinary Medicine , Shenyang Agricultural University , Shenyang , P. R. China.
2
b Sichuan Animal Science Academy , Chengdu , P. R. China.
3
c Academy of Animal Husbandry Science of Liaoning Province , Liaoyang , P. R. China.

Abstract

Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200‚ÄČnt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber. Taken together with methylation analysis, we showed that 5' regulatory region methylation of the lncRNA-000133 gene might be involved in its expression suppression in SHF of Cashmere goat. The ceRNA regulatory network showed that a rich and complex regulatory relationship between lncRNA-000133 and related miRNAs with their target genes. The overexpression of lncRNA-000133 led to a significant increasing in the relative expression of ET-1, SCF, ALP and LEF1 in dermal papilla cells suggesting that lncRNA-000133 appears to contribute the inductive property of dermal papilla cells.

KEYWORDS:

Cashmere goat; LncRNA-000133; SHF; dermal papilla cells; inductive property; regulatory network

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