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Hum Gene Ther Clin Dev. 2019 Jan 11. doi: 10.1089/humc.2018.177. [Epub ahead of print]

Knockdown of LINC02465 suppresses gastric cancer cell growth and metastasis via PI3K/AKT pathway.

Author information

1
Department of Gastroenterology, First People's Hospital of Yancheng City, YanchengYancheng, China , 224001 ; LiangHan_338@163.com.
2
Yancheng, China ; Yanping_H2@163.com.
3
Yancheng, China ; jianhuawang_123@163.com.
4
Yancheng, China ; Zhengjiang_Wdoctor@163.com.
5
Yancheng, China ; Hongmeihongmei_88@163.com.
6
Yancheng, China ; XudongWu_WXD@163.com.

Abstract

Gastric cancer (GC) is the second primary cause of cancer-associated mortality around the world. Long non-coding RNAs (lncRNAs) are critical modulators of multiple cellular processes, and their abnormal expression and/or function are related to a variety of diseases, including cancer. Nowadays, various lncRNAs have been unveiled to exert a functional role in GC, but more still remain to be identified since the therapies for GC patients are limited. Herein, we discover LINC02465, a novel recognized lncRNA, is upregulated and correlated with tumour size, tumour stage, lymph node metastasis and differentiation in gastric cancer. Besides, high LINC02465 level in GC patients is closely related to poor prognosis. Moreover, our findings reveal that LINC02465 silence suppresses cell proliferation and migration, invasion and EMT in vitro. Conversely, LINC02465 overexpression displays a completely opposite way. Meanwhile, LINC02465 inhibition also limits tumor growth in vivo. Mechanistically, LINC02465 inhibition inactivates PI3K/AKT signaling pathway and the activation of this pathway by 740Y-P reverses the inhibition effect of LINC02465 suppression on biological behaviors of GC cells. Taken together, LINC02465 is an oncogenic lncRNA which facilitates the tumorigenesis and progression of GC via PI3K/AKT pathway, demonstrating a novel effective therapeutic target and prognostic biomarker for GC patients.

PMID:
30632400
DOI:
10.1089/humc.2018.177

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