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Oncogenesis. 2019 Jan 9;8(1):4. doi: 10.1038/s41389-018-0110-2.

Cullin 5 is a novel candidate tumor suppressor in renal cell carcinoma involved in the maintenance of genome stability.

Author information

1
Section of Molecular Urooncology, Department of Urology, University of Heidelberg School of Medicine, Im Neuenheimer Feld 517, D-69120, Heidelberg, Germany.
2
Department of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 460, D-69120, Heidelberg, Germany.
3
Division of Solid Tumor Translational Oncology, West German Cancer Center (WTZ), Essen University Hospital, German Cancer Consortium (DKTK) at Essen/Düsseldorf, Hufelandstrasse 55, D-45147, Essen, Germany.
4
Division of Applied Functional Genomics, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, D-69120, Heidelberg, Germany.
5
Department of Urology, University of Heidelberg School of Medicine, Im Neuenheimer Feld 110, D-69120, Heidelberg, Germany.
6
Cancer Therapeutics Program, UPMC Hillman Cancer Center and Department of Pathology, University of Pittsburgh School of Medicine, UPMC Hillman Cancer Center, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA.
7
Section of Molecular Urooncology, Department of Urology, University of Heidelberg School of Medicine, Im Neuenheimer Feld 517, D-69120, Heidelberg, Germany. stefan.duensing@med.uni-heidelberg.de.
8
Department of Urology, University of Heidelberg School of Medicine, Im Neuenheimer Feld 110, D-69120, Heidelberg, Germany. stefan.duensing@med.uni-heidelberg.de.

Abstract

Clear cell renal cell carcinoma (ccRCC) is intimately associated with defects in ubiquitin-mediated protein degradation. Herein, we report that deficiency in the E3 ligase subunit cullin 5 (CUL5) promotes chromosomal instability and is an independent negative prognostic factor in ccRCC. CUL5 was initially identified in an RNA interference screen as a novel regulator of centrosome duplication control. We found that depletion of CUL5 rapidly promotes centriole overduplication and mitotic errors. Downregulation of CUL5 also caused an increase of DNA damage that was found to involve impaired DNA double-strand break repair. Using immunohistochemistry, CUL5 protein expression was found to be below detection level in the majority of RCCs. A re-analysis of the TCGA ccRCC cohort showed that a reduced CUL5 gene expression or CUL5 deletion were associated with a significantly worse overall patient survival. In conclusion, our results indicate that CUL5 functions as a novel tumor suppressor with prognostic relevance in ccRCC and is critically involved in the maintenance of genome stability.

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