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Int J Syst Evol Microbiol. 2019 Jan 10. doi: 10.1099/ijsem.0.003211. [Epub ahead of print]

Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment.

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2​Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA-CNRS-Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
1​Laboratory Molecules of Communication and Adaptation of Microorganisms (MCAM) UMR 7245 CNRS-MNHN), Muséum national d'Histoire naturelle, Centre national de la Recherche scientifique (CNRS), CP 54, 57 rue Cuvier, 75005 Paris, France.
3​Laboratory of Engineering of Macromolecular Systems (LISM), Aix Marseille University, UMR 7255 CNRS, 31 chemin Joseph Aiguier 13402 Marseille cedex 20, France.
4​Laboratory of Tropical and Mediterranean Symbioses (LSTM), Campus International de Baillarguet, 34398 Montpellier cedex 5, France.


A halophilic organism, SWO25T, was isolated from water sampled in Algeria at the salt lake (sebkha) of Ouargla. The novel strain stained Gram-negative, and cells were pleomorphic with a red pigmentation. Strain SWO25T grew optimally at 35-45 °C, at pH 6.0-8.0 and 0.05-0.25 M MgCl2 concentrations. Cells were extremely halophilic, with optimal growth at 4.3-5.1 M NaCl. The predominant membrane polar lipids were C20C20 glycerol diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and diglycosyl diether. The major respiratory menaquinone component was MK-8. Cells were highly tolerant to the presence of decane and isooctane in the growth medium. Chemotaxonomic properties supported the assignment of strain SWO25T to the genus Haloarcula. The DNA G+C content was 61.1mol%. DNA-DNA hybridization and phylogenetic analyses of the 16S rRNA and rpoB' genes showed that strain SWO25T is distinct from known Haloarcula species. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, we describe a novel species of the genus Haloarcula, for which the name Haloarculasebkhae sp. nov. is proposed. The type strain is SWO25T (=CIP 110583T=JCM 19018T).


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