Format

Send to

Choose Destination
Cell Rep. 2019 Jan 8;26(2):394-406.e5. doi: 10.1016/j.celrep.2018.12.044.

Single-Cell Analysis of Regional Differences in Adult V-SVZ Neural Stem Cell Lineages.

Author information

1
Department of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA.
2
Department of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA.
3
Biozentrum, University of Basel, 4056 Basel, Switzerland.
4
Department of Systems Biology, Columbia University Medical Center, New York, NY 10032, USA; Sulzberger Columbia Genome Center, Columbia University Medical Center, New York, NY 10032, USA; Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA. Electronic address: pas2182@cumc.columbia.edu.
5
Biozentrum, University of Basel, 4056 Basel, Switzerland. Electronic address: fiona.doetsch@unibas.ch.

Abstract

The ventricular-subventricular zone (V-SVZ) harbors adult neural stem cells. V-SVZ neural stem cells exhibit features of astrocytes, have a regional identity, and depending on their location in the lateral or septal wall of the lateral ventricle, generate different types of neuronal and glial progeny. We performed large-scale single-cell RNA sequencing to provide a molecular atlas of cells from the lateral and septal adult V-SVZ of male and female mice. This revealed regional and sex differences among adult V-SVZ cells. We uncovered lineage potency bias at the single-cell level among lateral and septal wall astrocytes toward neurogenesis and oligodendrogenesis, respectively. Finally, we identified transcription factor co-expression modules marking key temporal steps in neurogenic and oligodendrocyte lineage progression. Our data suggest functionally important spatial diversity in neurogenesis and oligodendrogenesis in the adult brain and reveal molecular correlates of adult NSC dormancy and lineage specialization.

KEYWORDS:

adult neural stem cell; adult neurogenesis; female; gliogenesis; male; regional heterogeneity; single cell; stem cell niche; ventricular-subventricular zone

PMID:
30625322
DOI:
10.1016/j.celrep.2018.12.044
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center