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Int J Syst Evol Microbiol. 2019 Jan 9. doi: 10.1099/ijsem.0.003215. [Epub ahead of print]

Polyphasic classification of the gifted natural product producer Streptomyces roseifaciens sp. nov.

Author information

1
1​Molecular Biotechnology, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands.
2
2​School of Natural and Environmental Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
3
3​Instituto de Recursos Naturales y Agrobiologia de Salamanca, Consejo Superior de Investigaciones Cientificas (IRNASACSIC), c/Cordel de Merinas 40-52, 37008 Salamanca, Spain.
4
4​Department of Microbial Ecology, Netherlands, Institute of Ecology (NIOO-KNAW), Droevendaalsteeg 10, Wageningen 6708 PB, The Netherlands.

Abstract

A polyphasic study was designed to establish the taxonomic status of a Streptomyces strain isolated from soil from the QinLing Mountains, Shaanxi Province, China, and found to be the source of known and new specialized metabolites. Strain MBT76T was found to have chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. The strain formed a distinct branch in the Streptomyces16S rRNA gene tree and was closely related to the type strains of Streptomyces hiroshimensis and Streptomycesmobaraerensis. Multi-locus sequence analyses based on five conserved house-keeping gene alleles showed that strain MBT76T is closely related to the type strain of S. hiroshimensis, as was the case in analysis of a family of conserved proteins. The organism was also distinguished from S. hiroshimensis using cultural and phenotypic features. Average nucleotide identity and digital DNA-DNA hybridization values between the genomes of strain MBT76T and S. hiroshimensis DSM 40037T were 88.96 and 28.4±2.3%, respectively, which is in line with their assignment to different species. On the basis of this wealth of data it is proposed that strain MBT76T (=DSM 106196T=NCCB 100637T), be classified as a new species, Streptomycesroseifaciens sp. nov.

PMID:
30625109
DOI:
10.1099/ijsem.0.003215

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