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Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7.

Transforming insect population control with precision guided sterile males with demonstration in flies.

Author information

1
Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, California, USA.
2
Division of Biostatistics and Epidemiology, School of Public Health, University of California, Berkeley, CA 94720, California, USA.
3
Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, California, USA. oakbari@ucsd.edu.
4
Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA 92093, California, USA. oakbari@ucsd.edu.

Abstract

The sterile insect technique (SIT) is an environmentally safe and proven technology to suppress wild populations. To further advance its utility, a novel CRISPR-based technology termed precision guided SIT (pgSIT) is described. PgSIT mechanistically relies on a dominant genetic technology that enables simultaneous sexing and sterilization, facilitating the release of eggs into the environment ensuring only sterile adult males emerge. Importantly, for field applications, the release of eggs will eliminate burdens of manually sexing and sterilizing males, thereby reducing overall effort and increasing scalability. Here, to demonstrate efficacy, we systematically engineer multiple pgSIT systems in Drosophila which consistently give rise to 100% sterile males. Importantly, we demonstrate that pgSIT-generated sterile males are fit and competitive. Using mathematical models, we predict pgSIT will induce substantially greater population suppression than can be achieved by currently-available self-limiting suppression technologies. Taken together, pgSIT offers to potentially transform our ability to control insect agricultural pests and disease vectors.

PMID:
30622266
PMCID:
PMC6325135
DOI:
10.1038/s41467-018-07964-7
[Indexed for MEDLINE]
Free PMC Article

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