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Stem Cell Res. 2019 Jan;34:101353. doi: 10.1016/j.scr.2018.11.014. Epub 2018 Dec 19.

Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family.

Author information

1
Monash Institute of Cognitive and Clinical Neurosciences and School of Psychological Sciences, Monash University, Wellington Road, Clayton, Victoria, Australia. Electronic address: janette.tong@monash.edu.
2
Monash Institute of Cognitive and Clinical Neurosciences and School of Psychological Sciences, Monash University, Wellington Road, Clayton, Victoria, Australia.
3
Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
4
Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603203, India.
5
Murdoch Children's Research Institute, Parkville, VIC, Australia; Royal Children's Hospital, Melbourne, VIC, Australia; Department of Pediatrics, University of Melbourne, Parkville, VIC, Australia; Department of Psychiatry, University of Melbourne, Parkville, VIC, Australia.
6
Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC 3010, Australia.

Abstract

Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.

PMID:
30622032
DOI:
10.1016/j.scr.2018.11.014
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