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Int J Mol Sci. 2019 Jan 4;20(1). pii: E164. doi: 10.3390/ijms20010164.

Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.

Chu Y1, Wu PY2,3, Chen CW4, Lyu JL5,6, Liu YJ7,8, Wen KC9, Lin CY10, Kuo YH11,12, Chiang HM13,14.

Author information

1
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. dooo517@outlook.com.
2
Department of Dermatology, China Medical University Hospital, Taichung 40402, Taiwan. wu.poyuan@gmail.com.
3
School of Medicine, China Medical University, Taichung 40402, Taiwan. wu.poyuan@gmail.com.
4
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. walnut0727@hotmail.com.
5
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. rain26842006@gmail.com.
6
Ph.D Program for Biotechnology Industry, China Medical University, Taichung 40402, Taiwan. rain26842006@gmail.com.
7
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. ella8175@gmail.com.
8
Ph.D Program for Biotechnology Industry, China Medical University, Taichung 40402, Taiwan. ella8175@gmail.com.
9
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. kcwen0520@mail.cmu.edu.tw.
10
Department of Biotechnology, Asia University, Taichung 41354, Taiwan. yihlin@asia.edu.tw.
11
Department of Biotechnology, Asia University, Taichung 41354, Taiwan. kuoyh@mail.cmu.edu.tw.
12
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. kuoyh@mail.cmu.edu.tw.
13
Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. hmchiang@mail.cmu.edu.tw.
14
Ph.D Program for Biotechnology Industry, China Medical University, Taichung 40402, Taiwan. hmchiang@mail.cmu.edu.tw.

Abstract

The skin provides an effective barrier against physical, chemical, and microbial invasion; however, overexposure to ultraviolet (UV) radiation causes excessive cellular oxidative stress, which leads to skin damage, DNA damage, mutations, and skin cancer. This study investigated the protective effects of N-phenethyl caffeamide (K36) from UVA damage on human epidermal keratinocytes. We found that K36 reduced UVA-induced intracellular reactive oxygen species (ROS) production and induced the expression of the intrinsic antioxidant enzyme heme oxygenase-1 (HO-1) by increasing the translocation of nuclear factor erythroid 2⁻related factor 2 (Nrf2). K36 could inhibit the phosphorylation of extracellular-signal-regulated kinase (ERK) and c-Jun N-terminal kinases (JNK) and reduce UVA-induced matrix metalloproteinase (MMP)-1 and MMP-2 overexpression; it could also elevate the expression of tissue inhibitors of metalloproteinases (TIMP). In addition, K36 ameliorated 8-hydroxy-2'-deoxyguanosine (8-OHdG) induced by UVA irradiation. Furthermore, K36 could downregulate the expression of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) and the subsequent production of nitric oxide (NO) and prostaglandin E₂ (PGE₂). Based on our findings, K36 possessed potent antioxidant, anti-inflammatory, antiphotodamage, and even antiphotocarcinogenesis activities. Thus, K36 has the potential to be used to multifunctional skin care products and drugs.

KEYWORDS:

8-hydroxy-2’-deoxyguanosine (8-OHdG); N-phenethyl caffeamide; heme oxygenase-1 (HO-1); nuclear factor erythroid 2–related factor 2 (Nrf2); photodamage; photoinflammation

PMID:
30621167
DOI:
10.3390/ijms20010164
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