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Int J Mol Sci. 2019 Jan 7;20(1). pii: E192. doi: 10.3390/ijms20010192.

The Fate of Transplanted Periodontal Ligament Stem Cells in Surgically Created Periodontal Defects in Rats.

Author information

1
Institute of Dental Research, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, Japan. iwasaki-k@cc.osaka-dent.ac.jp.
2
Department of Nanomedicine (DNP), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. iwasaki-k@cc.osaka-dent.ac.jp.
3
Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. iwasaki-k@cc.osaka-dent.ac.jp.
4
Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. akakperi@tmd.ac.jp.
5
Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. mizukin@umich.edu.
6
Kanagawa Dental University, Yokohama Clinic, 3-31-6, Tsuruya-cho, Kanagawa-ku, Yokohama, Kanagawa 221-0835, Japan. m.komaki@kdu.ac.jp.
7
Department of Comprehensive Reproductive Medicine, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. izumarch@gmail.com.
8
Department of Pediatrics and Developmental Biology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. cmorped@tmd.ac.jp.
9
Biomaterial Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 250-1, Wakashiba, Kashiwa-city, Chiba 277-0871, Japan. yokoyama-n4@mail.dnp.co.jp.
10
Biomaterial Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 250-1, Wakashiba, Kashiwa-city, Chiba 277-0871, Japan. ayame-h@mail.dnp.co.jp.
11
Biomaterial Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 250-1, Wakashiba, Kashiwa-city, Chiba 277-0871, Japan. Yamaki-K2@mail.dnp.co.jp.
12
Biomaterial Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 250-1, Wakashiba, Kashiwa-city, Chiba 277-0871, Japan. Tanaka-Y64@mail.dnp.co.jp.
13
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. kimurat.mbme@tmd.ac.jp.
14
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. kishida.mbme@tmd.ac.jp.
15
Biochemistry, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. t-watabe@umin.ac.jp.
16
Ochanomizu University, 2-1-1, Otsuka, Bunkyo-Ku, Tokyo 112-8610, Japan. morita.ikuo@ocha.ac.jp.

Abstract

Periodontal disease is chronic inflammation that leads to the destruction of tooth-supporting periodontal tissues. We devised a novel method ("cell transfer technology") to transfer cells onto a scaffold surface and reported the potential of the technique for regenerative medicine. The aim of this study is to examine the efficacy of this technique in periodontal regeneration and the fate of transplanted cells. Human periodontal ligament stem cells (PDLSCs) were transferred to decellularized amniotic membrane and transplanted into periodontal defects in rats. Regeneration of tissues was examined by microcomputed tomography and histological observation. The fate of transplanted PDLSCs was traced using PKH26 and human Alu sequence detection by PCR. Imaging showed more bone in PDLSC-transplanted defects than those in control (amnion only). Histological examination confirmed the enhanced periodontal tissue formation in PDLSC defects. New formation of cementum, periodontal ligament, and bone were prominently observed in PDLSC defects. PKH26-labeled PDLSCs were found at limited areas in regenerated periodontal tissues. Human Alu sequence detection revealed that the level of Alu sequence was not increased, but rather decreased. This study describes a novel stem cell transplantation strategy for periodontal disease using the cell transfer technology and offers new insight for cell-based periodontal regeneration.

KEYWORDS:

mesenchymal stem cells; periodontal disease; regeneration

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