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Inhal Toxicol. 2018 Sep - Oct;30(11-12):463-471. doi: 10.1080/08958378.2018.1554014. Epub 2019 Jan 8.

Changes in lipid profile parameters and PON1 status associated with L55M PON1 polymorphism, overweight and exposure to tobacco smoke.

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1
a Department of Biomedical and Environmental Analysis, Faculty of Pharmacy with Division of Laboratory Diagnostics , Wroclaw Medical University , Wrocław , Poland.

Abstract

OBJECTIVE:

The aim of our study was to investigate the effects of paraoxonase 1 (PON1) L55M polymorphism on the enzyme's activity and concentration in the serum as well as its association with lipid profile parameters in a group of healthy persons. We also evaluated the presence of PON1 L55M polymorphism in a group of subjects exposed to tobacco smoke and with overweight or obesity on those parameters.

METHODS:

Analysis of L55M polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (PCR - RFLP). Serum PON1 concentration and lipid profile parameters were assayed using commercial tests. PON1 activities were measured earlier elaborated procedures.

RESULTS:

We observed a statistically significant difference in HDL and PON1 concentration: the highest in the LL genotype and the lowest in the MM genotype with the LM genotype having an intermediate concentration. L55M polymorphism influence on PON1 arylesterase and phosphotriesterase activity, whereas PON1 lactonase activity did not differ in all polymorphic variant groups. Exposure to tobacco smoke and overweight or obesity additionally disorder above mentioned parameters. Overweight and obesity in LM and MM genotype could be associated with higher PON1 phosphotriesterase activity. It is also possible that MM genotype could be a determinant of smoking addiction.

CONCLUSIONS:

L55M polymorphism, like exposure to tobacco smoke and overweight, disorders PON1 status and lipid profile parameters; therefore, it could be a crucial risk factor for the development of many metabolic disorders.

KEYWORDS:

Paraoxonase; lipid profile; overweight; polymorphism; tobacco smoke

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