Format

Send to

Choose Destination
Methods Mol Biol. 2019;1938:155-168. doi: 10.1007/978-1-4939-9068-9_11.

In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes.

Author information

1
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
2
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. wonsuk.chung@kaist.ac.kr.

Abstract

Astrocytes eliminate unnecessary synapses, neural debris, and pathogenic proteins such as amyloid β plaque. Although the emerging evidences suggest that the phagocytic roles of astrocytes are critical in maintaining brain homeostasis during development as well as pathogenic conditions, the efficient assay for measuring phagocytic capacity and kinetics of astrocytes has been lacking. Here we present in vitro engulfment assay using purified astrocytes and synaptosomes. Based on imaging methods, either fluorescent or pH indicator-conjugated synaptosomes can be used in this assay.

KEYWORDS:

Astrocytes; Confocal microscope; Degradation; Engulfment; Live imaging; Phagocytosis; Synaptosomes; pH indicator

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center