Format

Send to

Choose Destination
Adv Exp Med Biol. 2018;1105:43-58. doi: 10.1007/978-981-13-2200-6_5.

Current Solution NMR Techniques for Structure-Function Studies of Proteins and RNA Molecules.

Author information

1
Biochemistry Department, University of Wisconsin-Madison, Madison, WI, USA. jmarkley@wisc.edu.

Abstract

We briefly review current technology for structure-function investigations of biological macromolecules in solution by nuclear magnetic resonance spectroscopy, which enable hybrid methods. An advantage of NMR is that biomolecules can be studied at atomic resolution under near physiological conditions where they are dynamically active. We outline stable isotope labeling strategies, NMR data collection methodology, and procedures for data analysis leading to structure-function information. We discuss issues related to NMR software and data deposition.

KEYWORDS:

Data deposition; Dynamics; Functional studies; NMR data collection strategies; NMR observables; NMR software packages; Spectral assignment; Stable isotope labeling; Structural restraints; Validation of NMR results

PMID:
30617823
DOI:
10.1007/978-981-13-2200-6_5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center