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Mol Cell Proteomics. 2019 Jan 7. pii: mcp.RA118.001257. doi: 10.1074/mcp.RA118.001257. [Epub ahead of print]

In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly.

Author information

1
Chinese Academy of Agricultural Sciences.
2
Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology.
3
Institute of Apicultural Research, Chinese Academy of Agricultural Science.
4
Institute of Apicultural Research, Chinese Academy of Agricultural Science, China.
5
Chinese Academy of Agricultural Sciences, Beijing.
6
Chinese Academy of Agricultural Science, China.
7
Institute of Apicultural Research,China, China.
8
Institute of Apicultural Research, Chinese Academy of Agricultural Science, China apislijk@163.com.

Abstract

Royal jelly (RJ) is a secretion of the hypopharyngeal glands (HGs) of honeybee workers. High royal jelly producing bees (RJBs), a stock of honeybees selected from Italian bees (ITBs), have developed a stronger ability to produce RJ than ITBs. However, the mechanism underpinning the high RJ-producing performance in RJBs is still poorly understood. We have comprehensively characterized and compared the proteome across the life span of worker bees between the ITBs and RJBs. Our data uncover distinct molecular landscapes that regulate the gland ontogeny and activity corresponding with age-specific tasks. Nurse bees (NBs) have a well-developed acini morphology and cytoskeleton of secretory cells in HGs to prime the gland activities of RJ secretion. In RJB NBs, pathways involved in protein synthesis and energy metabolism are functionally induced to cement the enhanced RJ secretion compared with ITBs. In behavior-manipulated RJB NBs, the strongly expressed proteins implicated in protein synthesis and energy metabolism further demonstrate their critical roles in the regulation of RJ secretion. Our findings provide a novel understanding of the mechanism consolidating the high RJ-output in RJBs.

KEYWORDS:

Energy metabolism; Molecular biology*; Protein Identification*; Protein Synthesis*; Ribosomes*

PMID:
30617159
DOI:
10.1074/mcp.RA118.001257
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