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Gene. 2019 Jan 4. pii: S0378-1119(18)31284-8. doi: 10.1016/j.gene.2018.12.027. [Epub ahead of print]

A planarian RPS3 homolog is critical to the modulation of planarian regeneration.

Author information

1
School of Life Sciences, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China.
2
School of Life Sciences, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China; Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou, Henan, China. Electronic address: zhangst@zzu.edu.cn.
3
School of Life Sciences, Zhengzhou University, No. 100 Science Avenue, Zhengzhou, Henan 450001, China; Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou, Henan, China. Electronic address: guoyn@zzu.edu.cn.

Abstract

Planarians are an important model for stem cell and regeneration biology, because they can regenerate any missing body structure in a short time. As an important component of ribosomes, ribosomal proteins can synthesize proteins and play a central role in cell cycle checkpoint, cell survival/senescence/apoptosis, and organismal growth and development. In this study, we identified and amplified the homologous gene of RPS3 in Dugesia japonica. Double-stranded RNA mediated RNAi revealed that when the Dj-RPS3 function was lost by planarians; they did not form blastemas and died 100%. Further investigation, confirmed that Dj-RPS3 was involved in regulating the proliferative and early differentiation of neoblasts.

KEYWORDS:

Dj-RPS3; Planarians; RNAi; Regeneration

PMID:
30615916
DOI:
10.1016/j.gene.2018.12.027

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