Format

Send to

Choose Destination
J Infect Dis. 2019 May 5;219(11):1755-1765. doi: 10.1093/infdis/jiz008.

Rapid Boosting of HIV-1 Neutralizing Antibody Responses in Humans Following a Prolonged Immunologic Rest Period.

Author information

1
Department of Pediatrics, Cincinnati Children's Hospital, Ohio.
2
Department of Surgery, Duke University Medical Center, Durham, North Carolina.
3
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.
4
Department of Biology, Emory University, Atlanta, Georgia.
5
Division of AIDS, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
6
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee.
7
Department of Medicine, University of Alabama at Birmingham, Cambridge, Massachusetts.
8
Novartis Vaccines, Cambridge, Massachusetts.

Abstract

BACKGROUND:

The durability and breadth of human immunodeficiency virus type 1 (HIV-1)-specific immune responses elicited through vaccination are important considerations in the development of an effective HIV-1 vaccine. Responses to HIV-1 envelope subunit protein (Env) immunization in humans are often described as short-lived.

METHODS:

We enrolled 16 healthy volunteers who had received priming with an HIV-1 subtype B Env vaccine given with MF59 adjuvant 5-17 years previously and 20 healthy unprimed volunteers. Three booster immunizations with a heterologous subtype C trimeric gp140 protein vaccine were administered to the primed group, and the same subtype C gp140 protein vaccination regimen was administered to the unprimed subjects.

RESULTS:

Binding antibodies and neutralizing antibodies to tier 1 viral isolates were detected in the majority of previously primed subjects. Remarkably, a single dose of protein boosted binding and neutralizing antibody titers in 100% of primed subjects following this prolonged immunologic rest period, and CD4+ T-cell responses were boosted in 75% of primed individuals.

CONCLUSIONS:

These results demonstrate that HIV-1 protein immunogens can elicit durable memory T- and B-cell responses and that strong tier 1 virus neutralizing responses can be elicited by a single booster dose of protein following a long immunologic rest period. However, we found no evidence that cross-clade boosting led to a significantly broadened neutralizing antibody response.

KEYWORDS:

HIV envelope; HIV vaccine; neutralization breadth; neutralizing antibodies

PMID:
30615119
PMCID:
PMC6775047
[Available on 2020-05-05]
DOI:
10.1093/infdis/jiz008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center