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Methods Mol Biol. 2019;1880:429-445. doi: 10.1007/978-1-4939-8873-0_27.

Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays.

Author information

1
Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen, Tübingen, Germany.
2
International Max Planck Research School "From Molecules to Organisms", Tübingen, Germany.
3
Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen, Tübingen, Germany. tassula.proikas-cezanne@uni-tuebingen.de.
4
International Max Planck Research School "From Molecules to Organisms", Tübingen, Germany. tassula.proikas-cezanne@uni-tuebingen.de.

Abstract

The fluorescence microscopy-based detection of intracellular LC3, p62, and/or WIPI punctate structures is a robust tool to monitor and assess macroautophagy/autophagy in single cells. This method was established for automated high-throughput/content analysis to reliably detect narrow differences in autophagy activity/capacity and to provide screening opportunities for biological and chemical libraries.

KEYWORDS:

Automated high-throughput analysis; Autophagy; LC3; WIPI; p62

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