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EMBO J. 2019 Jan 4. pii: e100240. doi: 10.15252/embj.2018100240. [Epub ahead of print]

A cis-acting bidirectional transcription switch controls sexual dimorphism in the liverwort.

Author information

1
Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
2
Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
3
Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
4
Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
5
School of Biological Sciences, Monash University, Melbourne, Vic., Australia.
6
Graduate School of Biostudies, Kyoto University, Kyoto, Japan tkohchi@lif.kyoto-u.ac.jp k-nakaji@bs.naist.jp.
7
Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan tkohchi@lif.kyoto-u.ac.jp k-nakaji@bs.naist.jp.

Abstract

Plant life cycles alternate between haploid gametophytes and diploid sporophytes. While regulatory factors determining male and female sexual morphologies have been identified for sporophytic reproductive organs, such as stamens and pistils of angiosperms, those regulating sex-specific traits in the haploid gametophytes that produce male and female gametes and hence are central to plant sexual reproduction are poorly understood. Here, we identified a MYB-type transcription factor, MpFGMYB, as a key regulator of female sexual differentiation in the haploid-dominant dioicous liverwort, Marchantia polymorpha MpFGMYB is specifically expressed in females and its loss resulted in female-to-male sex conversion. Strikingly, MpFGMYB expression is suppressed in males by a cis-acting antisense gene SUF at the same locus, and loss-of-function suf mutations resulted in male-to-female sex conversion. Thus, the bidirectional transcription module at the MpFGMYB/SUF locus acts as a toggle between female and male sexual differentiation in M. polymorpha gametophytes. Arabidopsis thaliana MpFGMYB orthologs are known to be expressed in embryo sacs and promote their development. Thus, phylogenetically related MYB transcription factors regulate female gametophyte development across land plants.

KEYWORDS:

Marchantia polymorpha ; R2R3 MYB‐type transcription factor; antisense transcription; lncRNA; sexual differentiation

PMID:
30609993
DOI:
10.15252/embj.2018100240
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