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FEBS Lett. 2019 Jan 4. doi: 10.1002/1873-3468.13325. [Epub ahead of print]

Generation of mesothelial progenitor-like cells from mouse induced pluripotent stem cells.

Author information

1
Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
2
Department of Lipid Signaling, National Center for Global Health and Medicine, Tokyo, Japan.
3
Hepato-Billary-Pancreatic Surgery Division, Artificial Organ and Transplantation Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
4
Department of Surgery, National Center for Global Health and Medicine, Tokyo, Japan.

Abstract

Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pluripotent stem cells (iPSCs) have the potential to be used for regenerative medicine. Here we have established a differentiation protocol for mouse iPSC-derived MPCs (miMPCs) via the exposure to defined factors, as well as purification using MPC-specific cell surface antigens. Furthermore, the miMPCs had the ability to suppress postoperative adhesion and facilitate liver regeneration. This is the first report highlighting the generation of functional miMPCs, which may offer potential for de novo cell therapy. This article is protected by copyright. All rights reserved.

KEYWORDS:

cell sheet; induced pluripotent stem cells; liver regeneration; mesothelial cells; postoperative adhesion

PMID:
30609020
DOI:
10.1002/1873-3468.13325

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