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Biochem Biophys Res Commun. 2019 Feb 5;509(2):603-610. doi: 10.1016/j.bbrc.2018.12.162. Epub 2018 Dec 31.

MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.

Author information

1
From the Department of Molecular Neurobiology, Shaanxi Normal University, Xi'an, 710119, China.
2
From the Department of Molecular Neurobiology, Shaanxi Normal University, Xi'an, 710119, China. Electronic address: ma_xinming@hotmail.com.

Abstract

MicroRNA-135a-5p has been reported to play a potential role in the generation of new neurons. However, the underlying targets of miR-135a-5p in regulating neuronal differentiation have been poorly understood. Our study recently has uncovered that Sox6 and CD44 genes were significantly downregulated during neuronal differentiation of P19 cells, a multipotent cell type. We then found that Sox6 directly bound to the promoter of CD44. Importantly, we identified Sox6 as a direct target of miR-135a-5p. Additionally, we demonstrated that miR-135a-5p is crucial for the neuronal differentiation of P19 cells. More significantly, we found that Sox6 overexpression could overturn miR-135a-5p-mediated neuronal differentiation and dendrite development. In conclusion, these findings indicated that miR-135a-5p/Sox6/CD44 axis provides an important molecular target mechanism for neurodifferentiation.

KEYWORDS:

CD44; Neuronal differentiation; P19 cells; Sox6; miR-135a-5p

PMID:
30606481
DOI:
10.1016/j.bbrc.2018.12.162

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