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Stem Cell Res. 2019 Jan;34:101356. doi: 10.1016/j.scr.2018.11.017. Epub 2018 Dec 18.

Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation.

Author information

1
Dept. of Microbiology, Tumor and Cell biology (MTC), Karolinska Institutet, Biomedicum B7, 17165 Stockholm, Sweden.
2
Dept. of Neuroscience, Karolinska Institutet, Biomedicum D7, 17165 Stockholm, Sweden.
3
Dept. of Women's and Children's health (KBH), Karolinska Institutet, 17177 Stockholm, Sweden.
4
Dept. of Microbiology, Tumor and Cell biology (MTC), Karolinska Institutet, Biomedicum B7, 17165 Stockholm, Sweden. Electronic address: Margareta.Wilhelm@ki.se.
5
Dept. of Neuroscience, Karolinska Institutet, Biomedicum D7, 17165 Stockholm, Sweden. Electronic address: Anna.Falk@ki.se.

Abstract

Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers.

PMID:
30605844
DOI:
10.1016/j.scr.2018.11.017
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