Format

Send to

Choose Destination
Stem Cell Res. 2019 Jan;34:101365. doi: 10.1016/j.scr.2018.101365. Epub 2018 Dec 20.

Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds.

Author information

1
Waisman Center, University of Wisconsin, Madison, WI, USA.
2
WiCell Research Institute, Madison, WI, USA.
3
Waisman Center, University of Wisconsin, Madison, WI, USA; Departments of Neuroscience, Neurology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA.
4
Waisman Center, University of Wisconsin, Madison, WI, USA; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA. Electronic address: bhattacharyy@waisman.wisc.edu.

Abstract

Seven human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts from three neonatal individuals using non-integrative reprogramming. Most control iPSCs are derived from adults, so these iPSCs meet the need for control iPSCs from young individuals. Donors were from different ethnicities and these lines provide unique genetic profiles. All iPSCs have normal karyotypes, express stem cell markers, and exhibit pluripotency, as assessed by capacity to differentiate into three germ layers. These lines are valuable to study human development, as age-matched controls for disorder-specific iPSCs, and as platforms for gene editing to control for age and ethnicity.

KEYWORDS:

Control iPSCs; Neonatal; Unaffected

PMID:
30605841
DOI:
10.1016/j.scr.2018.101365
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center