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ACS Synth Biol. 2019 Feb 15;8(2):463-465. doi: 10.1021/acssynbio.8b00440. Epub 2019 Jan 16.

A Standardized Synthetic Eucalyptus Transcription Factor and Promoter Panel for Re-engineering Secondary Cell Wall Regulation in Biomass and Bioenergy Crops.

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Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Genomics Research Institute (GRI) , University of Pretoria , Private Bag X28 , Pretoria 0002 , South Africa.
Laboratoire de Recherche en Sciences Végétales (LRSV) , Université Toulouse, UPS, CNRS, BP 42617 , F-31326 Castanet-Tolosan , France.
Department of Plant Biology and Genome Center , University of California , Davis , California 95616 , United States.
U.S. Department of Energy Joint Genome Institute , 2800 Mitchell Drive , Walnut Creek , California 94598 , United States.


Re-engineering of transcriptional networks regulating secondary cell wall formation may allow the improvement of plant biomass in widely grown plantation crops such as Eucalyptus. However, there is currently a scarcity of freely available standardized biological parts (e.g., Phytobricks) compatible with Type IIS assembly approaches from forest trees, and there is a need to accelerate transcriptional network inference in nonmodel biomass crops. Here we describe the design and synthesis of a versatile three-panel biological parts collection of 221 secondary cell wall-related Eucalyptus grandis transcription factor coding sequences and 65 promoters that are compatible with GATEWAY, Golden Gate, MoClo, and GoldenBraid DNA assembly methods and generally conform to accepted Phytobrick syntaxes. This freely available resource is intended to accelerate synthetic biology applications in multiple plant biomass crops and enable reconstruction of secondary cell wall transcriptional networks using high-throughput assays such as DNA affinity purification sequencing (DAP-seq) and enhanced yeast one-hybrid (eY1H) screening.


Eucalyptus; Phytobrick; secondary cell wall; transcriptional network


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