Format

Send to

Choose Destination
Endocr Metab Immune Disord Drug Targets. 2019 Jan 1. doi: 10.2174/1871530319666190102112844. [Epub ahead of print]

Testicular injury attenuated by rapamycin through induction of autophagy and inhibition of endoplasmic reticulum stress in streptozotocin-induced diabetic rats.

Author information

1
Shanxi Medical University, Taiyuan, Shanxi. China.
2
Department of Endocrinology, Second Hospital, Shanxi Medical University, Taiyuan, Shanxi. China.

Abstract

BACKGROUND AND OBJECTIVE:

This study investigated whether rapamycin has the protective effect on the testis of diabetic rats by regulating autophagy, endoplasmic reticulum stress, and oxidative stress.

METHODS:

Thirty male Sprague-Dawley rats were randomly divided into three groups: control, diabetic, and diabetic treated with rapamycin, which received gavage of rapamycin (2mg.kg-1.d-1) after induction of diabetes. Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65mg. Kg-1). All rats were sacrificed at the termination after 8 weeks of rapamycin treatment. The testicular pathological changes were determined by hematoxylin and eosin staining. The protein or mRNA expression of autophagy-related proteins (Beclin1, microtubule-associated protein light chain 3 (LC3), p62), ER stress marked proteins (CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP), caspase-12), oxidative stress-related proteins (p22phox, nuclear factor erythroid2-related factor 2 (Nrf2)) and apoptosis-related proteins (Bax, B cell lymphoma-2 (Bcl-2)) were assayed by western blot or real-time fluorescence quantitative PCR.

RESULTS:

There were significant pathological changes in the testes of diabetic rats. The expression of Beclin1, LC3, Nrf2, Bcl-2 were significantly decreased and p62, CHOP, caspase12, p22phox, and Bax were notably increased in the testis of diabetic rats (P <0.05). However, rapamycin treatment for 8 weeks significantly reversed the above changes in the testis of diabetic rats (P <0.05).

CONCLUSIONS:

Rapamycin appears to produce the protective effect on the testes of diabetic rats by inducing the expression of autophagy and inhibiting the expression of ER-stress, oxidative stress, and apoptosis.

KEYWORDS:

Autophagy; Endoplasmic reticulum stress; Oxidative stress; Rapamycin; Testis; Type 1 diabetes

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center