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Genes Genomics. 2019 Apr;41(4):459-465. doi: 10.1007/s13258-018-00778-3. Epub 2019 Jan 2.

Expression analysis of miR-221-3p and its target genes in horses.

Kim SW1,2, Jo A1,2, Im J1, Lee HE1,2, Kim HS3,4.

Author information

1
Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea.
2
Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea.
3
Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.
4
Institute of Systems Biology, Pusan National University, Busan, 46241, Republic of Korea. khs307@pusan.ac.kr.

Abstract

BACKGROUND:

A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.

OBJECTIVES:

We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.

METHODS:

We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).

RESULTS:

Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.

CONCLUSION:

Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.

KEYWORDS:

CDKN1C; Equus caballus; Expression analysis; Micro RNA 221-3p; Quantitative polymerase chain reaction (qPCR)

PMID:
30604147
DOI:
10.1007/s13258-018-00778-3
[Indexed for MEDLINE]

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