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Tissue Eng Regen Med. 2018 Jul 3;15(5):639-647. doi: 10.1007/s13770-018-0133-y. eCollection 2018 Oct.

Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics.

Yu NH#1, Chun SY#1, Ha YS2,3, Kim HT2,4, Kim DH5, Kim J6, Chung JW2,4, Lee JN2,4, Song PH7, Yoo ES2,3, Kim BS#2,3, Kwon TG#2,4.

Author information

1
1Biomedical Research Institute, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, 41944 Korea.
2
2Department of Urology, School of Medicine, Kyungpook National University, 130 Dongdeok-ro, Jung-gu, Daegu, 41944 Korea.
3
3Department of Urology, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, 41944 Korea.
4
4Department of Urology, Kyungpook National University Chilgok Hospital, 807 Hogukro, Buk-gu, Daegu, 41404 Korea.
5
5Department of Laboratory Animal Research Support Team, Yeungnam University Medical Center, 170 Hyunchung-ro, Nam-gu, Daegu, 42415 Korea.
6
Department of Pathology, Central Hospital, 480 Munsu-ro, Nam-gu, Ulsan, 44667 Korea.
7
7Department of Urology, College of Medicine, Yeungnam University, 170 Hyunchung-ro, Nam-gu, Daegu, 42415 Korea.
#
Contributed equally

Abstract

BACKGROUND:

The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions.

METHODS:

To establish optimal conditions, human amniotic fluid stem cells' (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × 107 cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells.

RESULTS:

Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells.

CONCLUSION:

Our findings can provide a foundation to optimization of conditions for stem cell transport.

KEYWORDS:

Amniotic fluid stem cell; Cell density; Cell transporting; Medium

Conflict of interest statement

The authors declare that they have no conflict of interest.This study was approved by the Ethics Committee of Kyungpook National University Hospital (IRB No. KNUH 2012-10-018). This study was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

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