Format

Send to

Choose Destination
Molecules. 2018 Dec 31;24(1). pii: E124. doi: 10.3390/molecules24010124.

Characterization of Purified Red Cabbage Anthocyanins: Improvement in HPLC Separation and Protective Effect against H₂O₂-Induced Oxidative Stress in HepG2 Cells.

Author information

1
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. fszjgsu@163.com.
2
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. linfubinwin@126.com.
3
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. daofeng@zjsu.edu.cn.
4
College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China. liangxrvicky@zjut.edu.cn.
5
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. wanglp155fs@sina.com.

Abstract

In this study, the chemical profiles and antioxidant activities of red cabbage anthocyanin (RCA)-enriched extract are evaluated. The effects of column temperature on the HPLC resolution of the RCAs are studied. The HPLC resolutions became better as the column temperature increased from 20 °C⁻45 °C. An optimized HPLC condition was achieved at 45 °C and used for the quantification and qualification of the RCAs. The anthocyanins in the enriched powder are all derivatives of cyanidin (268 ± 2 μg/mg), mainly with 19% nonacylated, 51% monoacylated, and 31% diacylated structures with ferulic, sinapic, p-coumaric, and caffeic acids characterized by HPLC-MS. The RCA extracts markedly reduced intracellular oxidative stress production by H₂O₂ on HepG2 cells and consequently ameliorated cell apoptosis and improved viability. The analytical method and cellular antioxidant activity demonstration of the RCAs will greatly facilitate their functional applications.

KEYWORDS:

HPLC; anthocyanins; intracellular oxidative stress; temperature

PMID:
30602654
PMCID:
PMC6337153
DOI:
10.3390/molecules24010124
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center