Format

Send to

Choose Destination
Cell. 1988 Dec 23;55(6):1159-69.

HIV expression strategies: ribosomal frameshifting is directed by a short sequence in both mammalian and yeast systems.

Author information

1
Department of Biochemistry, University of Oxford, England.

Abstract

The pol gene of the human immunodeficiency virus (HIV-1) is expressed as a gag:pol fusion, arising from a ribosomal frameshift that brings the overlapping, out-of-phase gag and pol genes into translational phase. In this study, we show that HIV frameshifting is mediated by a very short sequence in the viral RNA. We demonstrate the importance of a homopolymeric run within this sequence and conclude that HIV frameshifting is not dependent on stem-loop structures downstream from the frameshift site. Our analysis also indicates that the sequence requirements are identical in mammalian and yeast systems.

PMID:
3060262
DOI:
10.1016/0092-8674(88)90260-7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center