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Cell. 1988 Dec 23;55(6):1115-22.

GM-CSF and oncogene mRNA stabilities are independently regulated in trans in a mouse monocytic tumor.

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Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08540.


Regulation of mRNA turnover has emerged as an important control point in lymphokine and oncogene expression. We have studied a monocytic tumor in which activation of GM-CSF expression results from the constitutive stabilization of the normally short-lived GM-CSF mRNA. Linkage of the germ-line 3' untranslated region of the GM-CSF gene to a neo reporter gene demonstrated that mRNA stabilization is mediated by a tumor-specific trans-acting factor(s), rather than by an alteration of the GM-CSF gene itself. Significantly, similar fusions of the c-myc and c-fos 3' untranslated regions to neo yielded mRNAs that turned over rapidly in all cells, including the tumor cells. These results demonstrate that AU-rich mRNA turnover signals are recognized differentially in trans within the same cell.

[Indexed for MEDLINE]

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