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Mol Biol Cell. 2019 Jan 2:mbcE18070429. doi: 10.1091/mbc.E18-07-0429. [Epub ahead of print]

salto/CG13164 is required for sperm head morphogenesis in Drosophila.

Author information

1
Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69008, Lyon, France.

Abstract

Producing mature spermatozoa is essential for sexual reproduction in metazoans. Spermiogenesis involves dramatic cell morphological changes going from sperm tail elongation, nuclear reshaping to cell membrane remodeling during sperm individualization and release. The sperm manchette plays a critical scaffolding function during nuclear remodeling by linking the nuclear lamina to the cytoskeleton. Here, we describe the role of an uncharacterized protein in Drosophila, Salto/CG13164, involved in nuclear shaping and spermatid individualization. Salto has a dynamic localization during spermatid differentiation, being progressively relocated from the sperm nuclear dense body, which is equivalent to the mammalian sperm manchette, to the centriolar adjunct and acrosomal cap during spermiogenesis. salto null male flies are sterile and exhibit complete spermatid individualization defects. salto deficient spermatids show coiled spermatid nuclei at late maturation stages and stalled individualization complexes. Our work sheds light on a novel component involved in cytoskeleton based cell morphological changes during spermiogenesis.

PMID:
30601696
DOI:
10.1091/mbc.E18-07-0429

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